Protein engineers navigate toward more targeted therapeutics

Journal Reference:

  1. Fredrik Sadler, Ning Ma, Michael Ritt, Yatharth Sharma, Nagarajan Vaidehi, Sivaraj Sivaramakrishnan. Autoregulation of GPCR signalling through the third intracellular loop. Nature, 2023; DOI: 10.1038/s41586-023-05789-z

In a new study published in Nature, Sivaraj Sivaramakrishnan, a professor in the College of Biological Sciences, along with graduate student Fred Sadler and co-authors Michael Ritt and Yatharth Sharma, uncovered the role of the third intracellular loop in the GPCR’s signaling mechanism, suggesting the possibility of a more targeted approach to drug discovery and a paradigm shift for new therapeutics.

“Typical GPCR drugs act as on or off switches for cellular signaling outcomes,” said Sivaramakrishnan. “Drugs that leverage the loop effectively can act as signaling dimmer switches to more precisely control drug responses.”

The authors developed new biochemical and biophysical tools, combined with computational measurements by collaborators Ning Ma and Nagarajan Vaidehi at the City of Hope Cancer Center. They tracked how the third intracellular loop changes in shape, or conformation, through the receptor signaling process. In a breakthrough for the field, their data show that the loop acts as a kind of gate to ensure that receptors activate the correct type of G protein signaling at the right intensity.

“A key advantage of this loop is that it is highly unique, even among closely related receptors, making it an outstanding drug target,” said Sadler. “Developing drugs through this newly discovered mechanism would allow for far more targeted therapeutics.”

Funding was provided by the National Institutes of Health and the University of Minnesota Graduate School.

We want to say thanks to the writer of this short article for this amazing material

Protein engineers navigate toward more targeted therapeutics

Our social media profiles here as well as other pages related to them here.